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tissue pulverizer cellcrusher

Chemotherapeutic agent 5-fluorouracil increases survival
Each muscle was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher Cork Ireland) and half of the power was kept at -80°C for protein extraction (below). The powered muscle tissue was further homogenized using a PRO200 homogenizer (PRO Scientific Inc) and RNA was extracted with TRIzol reagent (Invitrogen).
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CELLCRUSHER TISSUE PULIVERIZER/NO ACCESORYFisher Sci
Manufacturer CELLCRUSHER TISSUE PULVERIZER B This ergonomically designed tissue pulverizer from Cellcrusher is used with liquid nitrogen to process varied tissue
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Novel Multiomics Profiling of Human Carotid
Samples were crushed while frozen using a tissue pulverizer (Cellcrusher Cork Ireland) and lysed and sonicated in a buffer containing 4 sodium dodecyl sulfate 25 mmol/l N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid pH 7.6. Lysates were centrifuged and supernatants collected for digestion.
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Deferoxamine-Soaked Suture Improves Angiogenesis and
Samples were frozen with liquid nitrogen and a tissue pulverizer (Cellcrusher) was used to homogenize the tissue. Homogenate from each sample was then centrifuged for 5 minutes at 13 000 rpm with 1 mL of 0.2 Triton X-100 cell lysis buffer (Sigma-Aldrich).
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Short inter‐set rest blunts resistance exercise‐induced
Approximately 25–30 mg of muscle tissue was powdered on dry ice using a Cellcrusher™ tissue pulverizer (Cellcrusher Ltd Cork Ireland) and prepared for Western blot analysis as described previously (Philp et al. 2015). Equal amounts of protein (30 μg) were boiled for 5 min in 1 Laemmli sample buffer and separated on 7.5–10 gels by
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IJMS Free Full-Text Inflammasome in ALS Skeletal
For tissue processing each skeletal muscle sample was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher Cork Ireland). Subsequently the tissue was divided into two parts one for the gene study and one for the protein study thus reducing the possibility of variation as it was the same animal in the
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Deferoxamine-Soaked Suture Improves Angiogenesis and
Samples were frozen with liquid nitrogen and a tissue pulverizer (Cellcrusher) was used to homogenize the tissue. Homogenate from each sample was then centrifuged for 5 minutes at 13 000 rpm with 1 mL of 0.2 Triton X-100 cell lysis buffer (Sigma-Aldrich).
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12-well dissociater-grinderGrinders / Potter tissue
CellCrusher tissue pulverizer. The strength of a world-class group The selection more than 100 000 products Technical and commercial expertise Tailor-made service Let s keep in touch Subscribe to the newsletter Welcome The Dutscher Group
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Mitochondrial uncoupler SHC517 reverses obesity in mice
Frozen liver tissue was powdered in liquid nitrogen using a tissue pulverizer (Cellcrusher Cork Ireland). Lipids were extracted using a modified version of the Folch method . In brief 25 mg of frozen powdered tissue was weighed out and vortexed in two parts chloroform (533 μL) and one part methanol (267 μL).
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Impact of the calcium form of β-hydroxy-β-methylbutyrate
Muscle (∼25 mg) was powdered on dry ice using a Cellcrusher™ tissue pulverizer (Cellcrusher Ltd Cork Ireland) and prepared as described . Equal amounts of protein (30 μg) were boiled for 5 min in 1 Laemmli buffer and separated on 10–15 gels by SDS-PAGE for 1 h.
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Chemotherapeutic agent 5-fluorouracil increases survival
Each muscle was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher Cork Ireland) and half of the power was kept at -80°C for protein extraction (below). The powered muscle tissue was further homogenized using a PRO200 homogenizer (PRO Scientific Inc) and RNA was extracted with TRIzol reagent (Invitrogen).
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Skeletal muscle mitochondrial function and exercise
Frozen gastrocnemius muscles were powdered using a Cellcrusher tissue pulverizer on dry ice and homogenized in 10-fold mass ice-cold lysis buffer 50 mM Tris 1 mM EDTA 1 mM EGTA 50 mM NaF 5 mM Na 4 P 2 O 7-10H 2 O 270 mM sucrose 1 M Triton-X 25 mM β-glycerophosphate 1 μM trichostatin A 10 mM nicotinamide 1 mM 1 4-dithiothreitol 1
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RANKL-independent osteoclastogenesis in the SH3BP2
After removing soft tissues head and distal end of the femur were cut off. The remaining part of the femur was flash frozen in liquid nitrogen and crushed in powder using a tissue pulverizer (Cellcrusher Limited Schull Ireland) for RNA isolation (Ribozol VWR Radnor PA USA). 2.7. Reverse transcription-quantitative PCR (RT-qPCR)
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Cryogenic Mechanical Crusher Crusher Mills Cone Crusher
Cellcrusher cryogenic tissue pulverizer The development of multifunctional crusher China the mechanical impact faster development of the crusher equipment High energy ball mill For the preparation of nano particles
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Identification of synovial fluid microRNA signature in
Total RNA isolation from tissue explants and miRNA detection in tissues and SF. Articular cartilage and synovium tissues were homogenized in liquid nitrogen using Cell crusher cryogenic tissue pulverizer (Cellcrusher Ireland). Total RNA was extracted from tissues (cartilage or synovium) using TRiZol reagent (Invitrogen Carlsbad CA) and
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Deferoxamine-Soaked Suture Improves Angiogenesis and
Samples were frozen with liquid nitrogen and a tissue pulverizer (Cellcrusher) was used to homogenize the tissue. Homogenate from each sample was then centrifuged for 5 minutes at 13 000 rpm with 1 mL of 0.2 Triton X-100 cell lysis buffer (Sigma-Aldrich).
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Skeletal muscle mitochondrial function and exercise
Frozen gastrocnemius muscles were powdered using a Cellcrusher tissue pulverizer on dry ice and homogenized in 10-fold mass ice-cold lysis buffer 50 mM Tris 1 mM EDTA 1 mM EGTA 50 mM NaF 5 mM Na 4 P 2 O 7-10H 2 O 270 mM sucrose 1 M Triton-X 25 mM β-glycerophosphate 1 μM trichostatin A 10 mM nicotinamide 1 mM 1 4-dithiothreitol 1
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Impact of the calcium form of β-hydroxy-β-methylbutyrate
Muscle (∼25 mg) was powdered on dry ice using a Cellcrusher™ tissue pulverizer (Cellcrusher Ltd Cork Ireland) and prepared as described . Equal amounts of protein (30 μg) were boiled for 5 min in 1 Laemmli buffer and separated on 10–15 gels by SDS-PAGE for 1 h.
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Mitochondrial uncoupler SHC517 reverses obesity in mice
Frozen liver tissue was powdered in liquid nitrogen using a tissue pulverizer (Cellcrusher Cork Ireland). Lipids were extracted using a modified version of the Folch method . In brief 25 mg of frozen powdered tissue was weighed out and vortexed in two parts chloroform (533 μL) and one part methanol (267 μL).
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IJMS Free Full-Text Inflammasome in ALS Skeletal
For tissue processing each skeletal muscle sample was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher Cork Ireland). Subsequently the tissue was divided into two parts one for the gene study and one for the protein study thus reducing the possibility of variation as it was the same animal in the
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Skeletal muscle mitochondrial function and exercise
Frozen gastrocnemius muscles were powdered using a Cellcrusher tissue pulverizer on dry ice and homogenized in 10-fold mass ice-cold lysis buffer 50 mM Tris 1 mM EDTA 1 mM EGTA 50 mM NaF 5 mM Na 4 P 2 O 7-10H 2 O 270 mM sucrose 1 M Triton-X 25 mM β-glycerophosphate 1 μM trichostatin A 10 mM nicotinamide 1 mM 1 4-dithiothreitol 1
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Short inter‐set rest blunts resistance exercise‐induced
Approximately 25–30 mg of muscle tissue was powdered on dry ice using a Cellcrusher™ tissue pulverizer (Cellcrusher Ltd Cork Ireland) and prepared for Western blot analysis as described previously (Philp et al. 2015). Equal amounts of protein (30 μg) were boiled for 5 min in 1 Laemmli sample buffer and separated on 7.5–10 gels by
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Mitochondrial uncoupler SHC517 reverses obesity in mice
Frozen liver tissue was powdered in liquid nitrogen using a tissue pulverizer (Cellcrusher Cork Ireland). Lipids were extracted using a modified version of the Folch method . In brief 25 mg of frozen powdered tissue was weighed out and vortexed in two parts chloroform (533 μL) and one part methanol (267 μL).
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Tissue Grinder Pulverizer With Liquid Nitrogen
Tissue Pulverizer Liquid Nitrogen Rna Extraction. Apr 04 2021 Frozen tissue samples are quickly and effectively pulverized and collected. The sample size range is 20 mg to 5 g. Fast cleaning The curved inner surfaces of the Cellcrusher have no corners facilitating easy cleaning.
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Chemotherapeutic agent 5-fluorouracil increases survival
Each muscle was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher Cork Ireland) and half of the power was kept at -80°C for protein extraction (below). The powered muscle tissue was further homogenized using a PRO200 homogenizer (PRO Scientific Inc) and RNA was extracted with TRIzol reagent (Invitrogen).
Chat Online
Chemotherapeutic agent 5-fluorouracil increases survival
Each muscle was pulverized in liquid nitrogen using a Cellcrusher cryogenic tissue pulverizer (Cellcrusher Cork Ireland) and half of the power was kept at -80°C for protein extraction (below). The powered muscle tissue was further homogenized using a PRO200 homogenizer (PRO Scientific Inc) and RNA was extracted with TRIzol reagent (Invitrogen).
Chat Online
Short inter‐set rest blunts resistance exercise‐induced
Approximately 25–30 mg of muscle tissue was powdered on dry ice using a Cellcrusher™ tissue pulverizer (Cellcrusher Ltd Cork Ireland) and prepared for Western blot analysis as described previously (Philp et al. 2015). Equal amounts of protein (30 μg) were boiled for 5 min in 1 Laemmli sample buffer and separated on 7.5–10 gels by
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